Effects of exosome-like vesicles on cumulus expansion in pigs in vitro

نویسندگان

  • Yuta MATSUNO
  • Asuka ONUMA
  • Yoshie A FUJIOKA
  • Kazuma YASUHARA
  • Wataru FUJII
  • Kunihiko NAITO
  • Koji SUGIURA
چکیده

Cell-secreted vesicles, such as exosomes, have recently been recognized as mediators of cell communication. A recent study in cattle showed the involvement of exosome-like vesicles in the control of cumulus expansion, a prerequisite process for normal ovulation; however, whether this is the case in other mammalian species is not known. Therefore, this study aimed to examine the presence of exosome-like vesicles in ovarian follicles and their effects on cumulus expansion in vitro in pigs. The presence of exosome-like vesicles in porcine follicular fluid (pFF) was confirmed by transmission electron microscopic observation, the detection of marker proteins, and RNA profiles specific to exosomes. Fluorescently labeled exosome-like vesicles isolated from pFF were incorporated into both cumulus and mural granulosa cells in vitro. Exosome-like vesicles were not capable of inducing cumulus expansion to a degree comparable to that induced by follicle-stimulating hormone (FSH). Moreover, exosome-like vesicles had no significant effects on the expression levels of transcripts required for the normal expansion process (HAS2, TNFAIP6, and PTGS2). Interestingly, FSH-induced expression of HAS2 and TNFAIP6 mRNA, but not of PTGS2 mRNA, was significantly increased by the presence of exosome-like vesicles; however, the degree of FSH-induced expansion was not affected. In addition, porcine exosome-like vesicles had no significant effects on the expansion of mouse cumulus-oocyte complexes. Collectively, the present results suggest that exosome-like vesicles are present in pFF, but they are not efficient in inducing cumulus expansion in pigs.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

P-109: Using SMAD2/3 Inhibitor to Investigate the Importance of GDF9 Signaling on Ovine Cumulus Expansion and Subsequent Embryonic Development

Background: Oocyte in vitro maturation success rate is still low in compareision to oocyte in vivo maturation. Thus prediction and improvement of oocyte competency are two critical issues in assisted reproductive technology. New insights into oocyte-cumulus cell regulatory loop may assist our optimization of in vitro maturation technique. Maturation and developmental competency of cumulus oocyt...

متن کامل

P-110: Effect of Increasing Amount of Oocyte Secreted Factors on Cumulus Expansion of Bovine Cumulus-Oocyte Complexes

Background: In vitro maturation is a good method to decrease cancer risk of superovulation by gonadotropin hormones. A paracrine effect of oocyte secretions on oocyte developmental competence is under investigation. Apart from oocyte maturation, ovulation in vivo requires a precise control of extracellular matrix modification. Cumulus cells secrete hyaluronan to form a muco-elastic extracellula...

متن کامل

P-29: Effects of Cumulus Cells on Survival, In vitro Maturation,Fertilization and Subsequent Developmental Capacity of Mouse Germinal Vesicle Stage Oocytes during Vitrification

Background: Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of cumulus cells on survival,in vitro maturation,fertilization and subsequent developmental capacity of mouse germinal vesicle stage oocytes during vitrification. Materials and...

متن کامل

The effects of activated-omental extract on nuclear and cytoplasmic in vitro maturation of rat oocytes

Objective: The role of growth factors, including vascular endothelial growth factor of activated omentum on mitosis is clearly known, though not on all the aspects of in vitro oocyte maturation. This study was designed to assess the effect of activated-omental extract (AOE) on in vitro maturation (IVM) of rat cumulus-oocyte complexes (COCs). Materials and Methods: In this experimental study, th...

متن کامل

P-32: Cumulus Cell Features and Nuclear ChromatinConfiguration of In vitro Matured CanineCOCs and the Influence of In vivo Serum ProgesteroneConcentrations of Ovary Donors

Background: The objectives of this study were: a) to observe the influence of cumulus investment expansion on the nuclear chromatin configuration of canine oocytes matured in vitro; b) to examine the relationship between cumulus cell (CC) expansion and its morphology after in vitro maturation (IVM); c) to ascertain the influence of in vivo serum progesterone (SP) concentrations of ovary donors ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 63  شماره 

صفحات  -

تاریخ انتشار 2017